I am trying to assemble a wheat genome (~15Gb) using ~20X ONT ultra-long reads (50kb N50). The command I used is: hifiasm --telo-m TTTAGGG -t 10 -l 0 -o XM.asm --ont UL.fastq.gz However, the job quickly exceeds 1TB of RAM and gets killed by the cluster. I noticed that in your benchmark table, the redwood genome (35.6Gb) was assembled with a maximum RAM of 699GB. Since my wheat genome is only about half the size of redwood, I am puzzled why it would exceed 1TB so quickly. Could you please advise what might be causing this excessive memory usage, and suggest ways to reduce memory consumption for large plant genomes like wheat?
I am trying to assemble a wheat genome (~15Gb) using ~20X ONT ultra-long reads (50kb N50). The command I used is: hifiasm --telo-m TTTAGGG -t 10 -l 0 -o XM.asm --ont UL.fastq.gz However, the job quickly exceeds 1TB of RAM and gets killed by the cluster. I noticed that in your benchmark table, the redwood genome (35.6Gb) was assembled with a maximum RAM of 699GB. Since my wheat genome is only about half the size of redwood, I am puzzled why it would exceed 1TB so quickly. Could you please advise what might be causing this excessive memory usage, and suggest ways to reduce memory consumption for large plant genomes like wheat?